Hybrid extracellular matrix microspheres for development of complex multicellular architectures

Demonstration of an extracellular matrix in multicellular tumor spheroids.

Multicellular spheroids of a human glioma cell line (U-118 MG) and a human thyroid cancer cell line ( HTh -7) were analyzed for the presence of extracellular matrix (ECM) using light microscopy, transmission electron microscopy, and indirect immunofluorescence staining for fibronectin, laminin, and collagen. These studies were supplemented by analyses of glycosaminoglycans using autoradiography...

Demonstration of an Extracellular Matrix in Multicellular Tumor Spheroids1

Multicellular spheroids of a human glioma cell line (U-118 MG) and a human thyroid cancer cell line (HTh-7) were analyzed for the presence of extracellular matrix (ECM) using light micros copy, transmission electron microscopy, and indirect immunofluorescence staining for fibronectin, laminin, and collagen. These studies were supplemented by analyses of glycosaminoglycans using autoradiography ...

Development of 177Lu-phytate Complex for Radiosynovectomy

Objective(s): In this work a new possible agent for radiosynovectomy has been targeted for articular pain palliation. Materials and Methods: Lu-177 of 2.6-3 GBq/mg specific activity was obtained by irradiation of natural Lu2O3 sample with thermal neutron flux of 4 × 1013 n.cm-2.s-1. The product was converted into chloride form which was further used for labeling of 177Lu-phytate complex and che...

Quantitative measurement of invadopodia-mediated extracellular matrix proteolysis in single and multicellular contexts.

Cellular invasion into local tissues is a process important in development and homeostasis. Malregulated invasion and subsequent cell movement is characteristic of multiple pathological processes, including inflammation, cardiovascular disease and tumor cell metastasis. Focalized proteolytic degradation of extracellular matrix (ECM) components in the epithelial or endothelial basement membrane ...

Vascular extracellular matrix and aortic development.